Coding

Part:BBa_K2481107:Design

Designed by: Erin Kelly   Group: iGEM17_Lethbridge_HS   (2017-10-27)


crtY and crtZ expression construct


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The native RBSs and coding sequences were assembled behind a T7 promoter that is inducible by IPTG in E. coli (BL219DE3) for overexpression.


Source

DNA synthesized based on Pantoes ananatis gene sequences.

References